soluble trail Search Results


recombinant human trail  (ProSci Incorporated)
90
ProSci Incorporated recombinant human trail
Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or <t>recombinant</t> <t>TRAIL</t> (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.
Recombinant Human Trail, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+trail/TRAIL%2C+Soluble+Recombinant+Protein/10__21767_slash_2476___1974__100008-74-29-32
Average 90 stars, based on 1 article reviews
recombinant human trail - by Bioz Stars, 2026-10
90/100 stars
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soluble trail  (Immunex Corporation)
90
Immunex Corporation soluble trail
Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or <t>recombinant</t> <t>TRAIL</t> (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.
Soluble Trail, supplied by Immunex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+trail/soluble+trail/pm11704827-173-112-113
Average 90 stars, based on 1 article reviews
soluble trail - by Bioz Stars, 2026-10
90/100 stars
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soluble trail/apo2l elisa kit  (ReproTech limited)
90
ReproTech limited soluble trail/apo2l elisa kit
Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or <t>recombinant</t> <t>TRAIL</t> (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.
Soluble Trail/Apo2l Elisa Kit, supplied by ReproTech limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+trail/soluble+trail+apo2l+elisa+kit/pmc05056653-55-2-6
Average 90 stars, based on 1 article reviews
soluble trail/apo2l elisa kit - by Bioz Stars, 2026-10
90/100 stars
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soluble recombinant human trail  (Enzo Biochem)
90
Enzo Biochem soluble recombinant human trail
Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or <t>recombinant</t> <t>TRAIL</t> (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.
Soluble Recombinant Human Trail, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+trail/soluble+recombinant+human+trail/pmc08212785-59-0-7
Average 90 stars, based on 1 article reviews
soluble recombinant human trail - by Bioz Stars, 2026-10
90/100 stars
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recombinant human soluble trail  (MBL Life science)
90
MBL Life science recombinant human soluble trail
Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or <t>recombinant</t> <t>TRAIL</t> (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.
Recombinant Human Soluble Trail, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+trail/recombinant+human+soluble+trail/pm21206980-34-0-22
Average 90 stars, based on 1 article reviews
recombinant human soluble trail - by Bioz Stars, 2026-10
90/100 stars
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soluble mouse trail  (Biomol GmbH)
90
Biomol GmbH soluble mouse trail
Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or <t>recombinant</t> <t>TRAIL</t> (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.
Soluble Mouse Trail, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+trail/soluble+mouse+trail/pmc00549384-128-3-11
Average 90 stars, based on 1 article reviews
soluble mouse trail - by Bioz Stars, 2026-10
90/100 stars
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100 ng/ml super killer trail (sktrail; soluble human recombinant)  (Enzo Biochem)
90
Enzo Biochem 100 ng/ml super killer trail (sktrail; soluble human recombinant)
Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or <t>recombinant</t> <t>TRAIL</t> (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.
100 Ng/Ml Super Killer Trail (Sktrail; Soluble Human Recombinant), supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+trail/100+ng+ml+super+killer+trail++sktrail++soluble+human+recombinant+/pm22231699-90-19-23
Average 90 stars, based on 1 article reviews
100 ng/ml super killer trail (sktrail; soluble human recombinant) - by Bioz Stars, 2026-10
90/100 stars
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soluble trail  (Genentech inc)
90
Genentech inc soluble trail
Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or <t>recombinant</t> <t>TRAIL</t> (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.
Soluble Trail, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+trail/soluble+trail/10__1128_slash_jvi__75__13__5930___5938__2001-52-0-9
Average 90 stars, based on 1 article reviews
soluble trail - by Bioz Stars, 2026-10
90/100 stars
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soluble, non-trimerized trail  (Genentech inc)
90
Genentech inc soluble, non-trimerized trail
Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or <t>recombinant</t> <t>TRAIL</t> (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.
Soluble, Non Trimerized Trail, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+trail/soluble++non+trimerized+trail/pm20450916-27-1-7
Average 90 stars, based on 1 article reviews
soluble, non-trimerized trail - by Bioz Stars, 2026-10
90/100 stars
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soluble trail protein dulanermin  (Genentech inc)
90
Genentech inc soluble trail protein dulanermin
Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or <t>recombinant</t> <t>TRAIL</t> (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.
Soluble Trail Protein Dulanermin, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+trail/soluble+trail+protein+dulanermin/10__1158_slash_1535___7163__mct___13___0645-171-19-24
Average 90 stars, based on 1 article reviews
soluble trail protein dulanermin - by Bioz Stars, 2026-10
90/100 stars
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soluble fusion protein trail-r2:fc  (Immunex Corporation)
90
Immunex Corporation soluble fusion protein trail-r2:fc
Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or <t>recombinant</t> <t>TRAIL</t> (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.
Soluble Fusion Protein Trail R2:Fc, supplied by Immunex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+trail/soluble+fusion+protein+trail+r2+fc/pm12817000-46-1-8
Average 90 stars, based on 1 article reviews
soluble fusion protein trail-r2:fc - by Bioz Stars, 2026-10
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recombinant soluble trail with a synthetic octapeptide ‘flag’ epitope appended to the nterminus  (Immunex Corporation)
90
Immunex Corporation recombinant soluble trail with a synthetic octapeptide ‘flag’ epitope appended to the nterminus
Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or <t>recombinant</t> <t>TRAIL</t> (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.
Recombinant Soluble Trail With A Synthetic Octapeptide ‘Flag’ Epitope Appended To The Nterminus, supplied by Immunex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soluble+trail/recombinant+soluble+trail+with+a+synthetic+octapeptide++flag%E2%80%99+epitope+appended+to+the+nterminus/pm09401075-25-0-35
Average 90 stars, based on 1 article reviews
recombinant soluble trail with a synthetic octapeptide ‘flag’ epitope appended to the nterminus - by Bioz Stars, 2026-10
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Image Search Results


Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or recombinant TRAIL (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.

Journal: Reproductive Immunology: Open Access

Article Title: NF-kB Regulation in T-cells in Pregnancy is Mediated via Fas/FasL Interactions: The Signal for which is Derived from Exosomes Present in Maternal Plasma

doi: 10.21767/2476-1974.100008

Figure Lengend Snippet: Figure 2: Factors present in maternal plasma can induce apoptosis in Jurkat T-cells. A) Jurkat T-cells were cultured in the presence of Fas activating antibody CH11 (0.625 µg/mL), or recombinant TRAIL (3 ng/mL) and the level of early (DilC1(5)-PI-) and late (DilC1(5)-PI+) apoptosis determined by flow cytometry. Flow charts are representative of 3 separate experiments with similar results. B) Jurkat T-cells were cultured in the presence of 20% (v/v) non-pregnant (NP) or pregnant (P) plasma from individual patients for 72 hrs and the apoptosis determined. Pooled data from 5 individual patients (NP and P15-19) is indicated in the bar graph. C) Jurkat T-cells were pre-incubated for 1 hr in the presence of ZB4 (500 ng/mL) or anti-TRAIL (0.8 ng/mL) and subsequently cultured for 72 hrs in the presence of 20% (v/v) NP or P plasma. Apoptosis was determined by Flow cytometry. The level of early (DilC1(5)-PI-) apoptosis is shown on the graph where n=4 for both NP and P samples. *p<0.05 relative to NP plasma.

Article Snippet: To assess the role of FasL and TRAIL activation in regulating p65 expression and apoptosis cells were incubated in the presence of the Fas activating antibody CH11 (Millipore) or recombinant human TRAIL (ProSci) at concentrations stated in the figure legends.

Techniques: Clinical Proteomics, Cell Culture, Recombinant, Flow Cytometry, Incubation

Figure 3: Factors present in maternal plasma can induce suppression of p65 in T-cells; A) Jurkat T-cells were cultured in the presence of increasing concentrations of Fas activating antibody CH11 or recombinant TRAIL and the level of p65 determined. Blot is representative of 3 separate experiments with similar results; B) Jurkat T-cells were cultured in the presence of CH11 (0.625 µg/mL) ±500 ng/mL Fas inactivating antibody ZB4 or mIgG control or IgM control (0.625 µg/mL) or recombinant TRAIL (3 ng/mL) ±80 ng/mL anti-TRAIL or mIgG control or BSA (3 ng/mL) and the level of p65 determined. Blot is representative of 3 separate experiments with similar results; C) Jurkat T-cells were cultured in the presence of CH11 (0.625 µg/mL) ±500 ng/mL Fas inactivating antibody ZB4 or mIgG control or IgM control (0.625 µg/mL) and the level of p65 and CD3ζ determined in cell lysates. Blot is representative of 3seperate experiments each showing similar results.

Journal: Reproductive Immunology: Open Access

Article Title: NF-kB Regulation in T-cells in Pregnancy is Mediated via Fas/FasL Interactions: The Signal for which is Derived from Exosomes Present in Maternal Plasma

doi: 10.21767/2476-1974.100008

Figure Lengend Snippet: Figure 3: Factors present in maternal plasma can induce suppression of p65 in T-cells; A) Jurkat T-cells were cultured in the presence of increasing concentrations of Fas activating antibody CH11 or recombinant TRAIL and the level of p65 determined. Blot is representative of 3 separate experiments with similar results; B) Jurkat T-cells were cultured in the presence of CH11 (0.625 µg/mL) ±500 ng/mL Fas inactivating antibody ZB4 or mIgG control or IgM control (0.625 µg/mL) or recombinant TRAIL (3 ng/mL) ±80 ng/mL anti-TRAIL or mIgG control or BSA (3 ng/mL) and the level of p65 determined. Blot is representative of 3 separate experiments with similar results; C) Jurkat T-cells were cultured in the presence of CH11 (0.625 µg/mL) ±500 ng/mL Fas inactivating antibody ZB4 or mIgG control or IgM control (0.625 µg/mL) and the level of p65 and CD3ζ determined in cell lysates. Blot is representative of 3seperate experiments each showing similar results.

Article Snippet: To assess the role of FasL and TRAIL activation in regulating p65 expression and apoptosis cells were incubated in the presence of the Fas activating antibody CH11 (Millipore) or recombinant human TRAIL (ProSci) at concentrations stated in the figure legends.

Techniques: Clinical Proteomics, Cell Culture, Recombinant, Control